Part:BBa_K4046500
cLuc (Cypridina luciferase)
Cypridina luciferase is an enzyme that plays an important role in bioluminescence. In order to do this, it cleaves its substrate, vargulin, which produces a luminescent molecule. cLuc that is expressed in cells can be secreted into the culturing media, which allows it to be used as a dynamic reporter for the concentration of the compound of interest.
In order to test the expression of this gene, we created two different plasmids. One contained the cLuc gene downstream of a CMV promoter, and the other one contained the cLuc gene downstream of a hUBC promoter.
These constructs were transfected into HEK cells, and cell media samples were tested for luciferase data after 2 days using vargulin. Overall, the results demonstrate that the cLuc gene behaves as expected, as it induces luminescence activity when acting on its vargulin substrate.
Figure 1: Luminescence assay results for cLuc gene with CMV promoter
Figure 2: Luminescence assay results for cLuc gene with hUBC promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 598
Illegal BglII site found at 657 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |